Reversible Cyclic Thermal Inactivation of Oligopeptidase B from Serratia proteamaculans
نویسندگان
چکیده
منابع مشابه
Quorum-sensing-directed protein expression in Serratia proteamaculans B5a.
N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-produci...
متن کاملTransglycosylation by chitinase D from Serratia proteamaculans improved through altered substrate interactions.
We describe the improvement of transglycosylation (TG) by chitinase D from Serratia proteamaculans (SpChiD). The SpChiD produced a smaller quantity of TG products for up to 90 min with 2 mm chitotetraose as the substrate and subsequently produced only hydrolytic products. Of the five residues targeted at the catalytic center, E159D resulted in substantial loss of both hydrolytic and TG activiti...
متن کاملSynthesis of long-chain chitooligosaccharides by a hypertransglycosylating processive endochitinase of Serratia proteamaculans 568.
We describe the heterologous expression and characterization of a 407-residue single-domain glycosyl hydrolase family 18 chitinase (SpChiD) from Gram-negative Serratia proteamaculans 568 that has unprecedented catalytic properties. SpChiD was optimally active at pH 6.0 and 40 °C, where it showed a K(m) of 83 mg ml(-1), a k(cat) of 3.9 × 10(2) h(-1), and a k(cat)/K(m) of 4.7 h mg(-1) ml(-1) on c...
متن کاملReversible inactivation of dehydrogenases.
1. The reversible dissociation of lactic and malic dehydrogenases has been studied in detail and compared with the dissociation properties of triosephosphate dehydrogenase, a-glycerophosphate dehydrogenase, and liver alcohol dehydrogenase. 2. Evidence is presented for the existence of an intermediate form of lactic dehydrogenase with catalytic properties altered during reactivation. 3. The tech...
متن کاملInverse relationship between chitobiase and transglycosylation activities of chitinase-D from Serratia proteamaculans revealed by mutational and biophysical analyses
Serratia proteamaculans chitinase-D (SpChiD) has a unique combination of hydrolytic and transglycosylation (TG) activities. The TG activity of SpChiD can be used for large-scale production of chito-oligosaccharides (CHOS). The multiple activities (hydrolytic and/or chitobiase activities and TG) of SpChiD appear to be strongly influenced by the substrate-binding cleft. Here, we report the unique...
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ژورنال
عنوان ژورنال: Acta Naturae
سال: 2018
ISSN: 2075-8251
DOI: 10.32607/20758251-2018-10-2-65-70